ABSTRACT
Mucoadhesive microspheres are drug delivery system intended for targeting a drug to particular region. Etodolac is a Non-steroidal anti-inflammatory drug. The objective of the present work was to develop sustain release of Etodolac loaded mucoadhesive microspheres due to relatively short residence time in the GIT tract before elimination. Mucoadhesive microspheres were prepared by non-aqueous solvent evaporation method using Carbopol 974P, HPMC K100M and HPMC K4M as the polymers. Central composite design was selected for the development of the formulation. Evaluation parameters like particle size, entrapment efficiency, shape and surface morphology, degree of swelling, drug content, in-vitro drug release study and in-vitro drug release study was done. Based on the results obtained from the preliminary formulations three optimized formulations were designed. The percentage mucoadhesion and swelling index of the optimized formulation was found in the range of 66-70 % and 82.50-83.84 % respectively. Optimized formulation releases 90.94% to 92.11% of drug at the end of 10 hours and follows zero order release kinetics.
ABSTRACT
Cancer is one of the most life threatening diseases and serious health problem in both developing and developed countries. Many synthetic and chemotherapeutic agents used in cancer therapy are having low bioavailability and involve the risk of life threatening host toxicity. Modern researchers are increasingly showing interest toward the improvement of bioavailability of a large number of drugs by addition of various herbs with bioenhancing properties. In oral drug delivery system, the co-administration of therapeutic agents with natural compounds possessing absorption improving activities, has also garnered great interest. Hence the present study was conducted to evaluate the anti-tumor activity of Mercaptopurine in combination with Trikatu and Gomutra. 20- Methylcholanthrene a Polycyclic aromatic hydrocarbons was used to induce tumor in albino mice. Haematological and endogenous antioxidant parameters were evaluated in the study. Individual treatment with Mercaptopurine (5mg/kg) and trikatu (100mg/kg) significantly restored the altered haematological and antioxidant parameters to normal values. Even Mercaptopurine (2.5mg/kg) at its sub therapeutic dose showed equivalent effects as that of therapeutic dose of Mercaptopurine (5mg/kg) when it was co administered along with trikatu compared to the positive tumor control group.
ABSTRACT
In the present study, Uvaria narum (Annonaceae) leaves were evaluated for its possible hepatoprotective potential. Elevation in hepatic biomarkers like SGPT, SGOT, ALP, bilirubin & other biochemical parameters like Cholesterol, triglycerides, urea & tissue LPO, where as decrease in total protein, albumin, glucose & tissue GSH, CAT, SOD was observed in CCl4 induced liver toxicity. Treatment with Silymarin, UNLE-200 and UNLE -400 resorted the altered hepatic biomarkers and biochemical parameters significantly in dose dependent manner. The biochemical observations were supplemented by histopathological examinations of liver sections. Relative organ weights were also reduced dose dependently in extract treated rats. Overall observations indicates that UNLE exerted remarkable hepatoprotective efficacy against CCl4 induced liver damage were comparable to reference standard Silymarin (100mg/kg) that is, may be due to its augmenting endogenous antioxidant mechanisms. These findings suggest the use of plant for treatment of liver diseases in oriental traditional medicine.
ABSTRACT
The present work has been designed to evaluate the potency of anti-inflammatory activity of different fractions of ethanolic extract of Achyranthes aspera leaves. Carrageenan induced rat paw oedema method was used for screening. Ethanolic, ethyl acetate and hexane fraction was screened among which ethyl acetate fraction was found to be most potent one with percentage inhibition of 50, 74, 84, 86% at 1st to 4th hour respectively. By this experiment it seems that leaves of Achyranthes aspera can be used for the treatment of acute inflammation.
ABSTRACT
In this study, the scavenging response of tetrahydrocurcumin (THC) was evaluated by using series of in vitro models of chemicals compared with standard antioxidant Trolox and curcumin. The THC exhibited its radical scavenging effect in concentration dependent manner on super oxide, hydroxyl radicals, ferric ions, 1,1-diphenyl, 2-picryl hydrazyl (DPPH) and 2,2- azinobis-(3-ethylbenzothiazoline-6-sulphonate) (ABTS). The THC was also evaluated for its inhibitory response on membrane lipid peroxidation by thiobarbituric acid reactive substances (TBARS) using rat liver homogenate. The present study shows that THC is a good antioxidant compared to the standard antioxidant, Trolox. Although, THC is less active than curcumin in many models, the increased superoxide scavenging and decreased ability to reduce ferric ions offers interesting advantages over curcumin. The THC was also effective in preventing membrane lipid peroxidation induced by FeSO4/ascorbate in concentration dependent manner.